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    Centrifuges, Multi-purpose Centrifuges, Micro-centrifuges

    Refrigerated, heated, vacuum-assisted, general-purpose ............


    We sell, supply, service and carry spare parts for these products.
    All equipment servicing to manufacturer’s specs by Eppendorf factory certified technicians. Calibration, repair, service, spare parts, Compliance Covering - IQ/OQ/PQ, calibration and validations. On-site repairs are available for customers in Sydney, Melbourne and Brisbane. For other areas/States, we offer a workshop service or spare parts.

    The list Best Sellers Our range history

    Centrifuges, Multi-purpose Centrifuges, Micro-centrifuges ~ list of Centrifuges we supply and service



    presenting our complete List of Centrifuges....

    POCD Scientific Laboratory Supplies offer a wide range of Eppendorf centrifuges and Tharmac Cytocentrifuges for nearly every market: ambient centrifuges and refrigerated models; Centrifuges for research, clinical trials, routine pathology, blood separation with, for example, Vacutainers in the main lab or at a collection centre. We will gladly install and train your staff onsite.

    POCD Scientific supply, install and service the Eppendorf Centrifuges: With adaptors from 0.2 ml tubes through to 500ml flasks, we can provide a centrifuge suited to your precise requirements.

    Micro Centrifuges / Microcentrifuges / Micofuges
     
    Eppendorf MiniSpin & MiniSpin   Eppendorf MiniSpin & MiniSpin plus Personal Micro Centrifuge
    compact and easy to use yet powerful enough for a wide range of molecular biology separations and quick spins

    Eppendorf Microcentrifuge Model 5418 Eppendorf Microcentrifuge Model 5418R   Eppendorf Microcentrifuge Model 5418 and 5418R (Refrigerated)
    compact 18-place centrifuge for labs with fewer samples but higher demand in quality and performance

    Eppendorf Microcentrifuge Model 5424 Eppendorf Microcentrifuge Model 5424R   Eppendorf Microcentrifuge Model 5424 and 5424R (Refrigerated)
    highly advanced 24-place models that are unbelievably quiet and equipped with a number of innovative features

    Eppendorf Microcentrifuge Model 5427R (Refrigerated)   Eppendorf Microcentrifuge Model 5427R (Refrigerated)
    only available as a refrigerated centrifuge, creates a bridge between the 5424R and the 5430R. Its main feature is its ability to spin 48x 1.5ml tubes.

    Eppendorf Microcentrifuge Model 5430 Eppendorf Microcentrifuge Model 5430R   Eppendorf Microcentrifuge Model 5430 and 5430R (Refrigerated)
    all-round 30 place air-cooled centrifuges are incomparably fast suitable for a wide range of applications due to the extensive choice of 8 different rotors
     
    General Purpose Centrifuges
     
    Eppendorf Centrifuge Model 5702   Eppendorf Centrifuge Model 5702
    compact, quiet, high-quality centrifuge that provides exceptional value for low-speed centrifugation in the low- to mid-throughput range

    Eppendorf Refrigerated Centrifuge Model 5702 R   Eppendorf Refrigerated Centrifuge Model 5702 R
    has all the product characteristics you will need for outstanding refrigerated centrifugation

    Eppendorf Temperature-controlled Centrifuge Model 5702 RH   Eppendorf Temperature-controlled Centrifuge Model 5702 RH
    unique in its temperature control capacity that can accurately maintain temperatures higher than room temperature - cells or cell cultures can be centrifuged in a constrained thermal environment
     
    Multipurpose Centrifuges
     
    Eppendorf Multipurpose Centrifuge Models 5804 Eppendorf Multipurpose Centrifuge Models 5804R   Eppendorf Multipurpose Centrifuge Models 5804 & 5804R (Refrigerated)
    feature powerful, maintenance-free motors, and they accommodate a variety of rotors for unparalleled application versatility

    Eppendorf Multipurpose Centrifuge Model 5810 Eppendorf Multipurpose Centrifuge Model 5810R   Eppendorf Multipurpose Centrifuge Model 5810 & 5810R (Refrigerated)
    three centrifuges in one: a high capacity general purpose centrifuge for cell harvesting; a high-speed centrifuge for separating cell lysates; and a micro centrifuge for DNA precipitations
     
    Cell preparation Cytocentrifuges
     
    Tharmac Cellspin I cell preparation Cytocentrifuge   Tharmac Cellspin I cell preparation Cytocentrifuge
    the leading system for economically producing cell preparations featuring the largest selection of “Quickchange” rotors and the largest selection of consumables for all applications with the proven monolayer filter card system.

    Tharmac Cellspin II cell preparation Cytocentrifuge (Refrigerated)   Tharmac Cellspin II cell preparation Cytocentrifuge (Refrigerated)
    the leading system for economically producing temperature-controlled (refrigerated) cell preparations featuring the largest selection of “Quickchange” rotors and the largest selection of consumables for all applications with the proven monolayer filter card system.
     
    Cytocentrifuge cell preparation Consumabless
     
      Cellfunnel, ECOfunnel and EASYfunnel consumables
    Standard, ECO and EASY Cellfunnel disposable and reuseable systems for cell preapration with the Tharmac Cellspin range also fit the Shandon Cytospin
     
    Centrifuge Accessories
     
    Genuine Eppendorf Micro Test Tubes   Genuine Eppendorf Micro Test Tubes
    perfect sealing qualities, excellent chemical stability and optimal construction materials guarantee unbeatable resistance to centrifugal force
     
    Vacuum Centrifugation
     
    Eppendorf Vacufuge Concentrator plus   Eppendorf Vacufuge Concentrator plus
    heating technology optimizes the evaporation process so you can be assured of quick, gentle and efficient vacuum concentration of your samples such as DNA/RNA, proteins.



    Centrifuges and Refrigerated 'fuges for research, clinical trials, routine pathology, blood separation with, for example, Vacutainers in the main lab or at a collection centre.
     
    We will gladly install and train your staff onsite.


    Centrifuges, Multi-purpose Centrifuges, Micro-centrifuges ~ Our top-of-the-range Best Sellers



    presenting our Best-selling ambient and Refrigerated Centrifuges....

    Micro-Centrifuge
    Model 5424
    Model 5424 Refrigerated
    Eppendorf Microcentrifuge Model 5424 Eppendorf Microcentrifuge Model 5424R A highly advanced 24-place model is unbelievably quiet and equipped with a number of innovative features. The Centrifuge 5424 and the Refrigerated Centrifuge 5424R set new standards for silence, speed and simplicity.

    Compact Benchtop Centrifuge
    Model 5702
    Model 5702R Refrigerated
    Model 5702RH Refrigerated & Heated
    Eppendorf Centrifuge Model 5702 A quiet, high-quality centrifuge that provides exceptional value for low-speed centrifugation in the low- to mid-throughput range. There are refrigerated and tepmerature controlled (heated) models available.

    Multi-purpose Centrifuge
    Model 5810
    Model 5810R Refrigerated
    Eppendorf Multipurpose Centrifuge Model 5810 Eppendorf Multipurpose Centrifuge Model 5810R These feature powerful, maintenance-free motors, and they accommodate a variety of rotors for unparalleled application versatility. They are three centrifuges in one: a high capacity general purpose centrifuge for cell harvesting; a high-speed centrifuge for separating cell lysates; and a micro centrifuge for DNA precipitations. There are refrigerated and non-refrigerated (ambient) models.





    Centrifuges and Refrigerated 'fuges for research, clinical trials, routine pathology, blood separation with, for example, Vacutainers in the main lab or at a collection centre.
     
    We will gladly install and train your staff onsite.





    Centrifuges, Multi-purpose Centrifuges, Micro-centrifuges ~ presenting our range



    presenting our range of Centrifuges....

         
     
    Eppendorf Centriguges Minispin and Minispin plus Eppendorf Centriguges 5418 and 5418R Eppendorf Centriguges 5424 and 5424R Eppendorf Centriguges 5424 and 5424REppendorf Centriguges 5430 and 5430R Eppendorf Centriguge 5702 Eppendorf Centriguge 5702R Eppendorf Centriguge 5702RH Eppendorf Centriguges 5804 and 5804R Tharmac Cellspin I & II Eppendorf Centriguges 5810 and 5810REppendorf Vacuum Concentrator
     
     
    previous slide button  click to toggle slide show next slide button
     
      click image to visit product page  





    Centrifuges and Refrigerated 'fuges for research, clinical trials, routine pathology, blood separation with, for example, Vacutainers in the main lab or at a collection centre.
     
    We will gladly install and train your staff onsite.




    Centrifuges, Multi-purpose Centrifuges, Micro-centrifuges ~ a brief history of Centrifugation



    Centrifuges today

    Eppendorf 5702 Centrifuge The smallest capacity centrifuge offers a fixed angle rotor for 12 X 1.5/2.0 ml tubes, with adaptors for 0.2ml, 0.4ml & 0.5ml microtubes. All rotors are autoclavable. Max RPM of 14,500 and max G’s of 14,000.

    Next size up is a centrifuge with a 24-place rotor for 1.5ml or 2.0ml tubes and same adaptors for microtubes with the standard rotor. Special rotor for Aerosol tight centrifugation available. Max G’s of 16,100. A larger brother centrifuge is available with adjustable ramp rates and standard 30 place rotor. A special fixed angle Hi G PCR rotor for 24 x 1.5/2.0ml tubes at 25000 G’s is available for a very economical price.

    A very reliable and flexible centrifuge with a small footprint is available with the capacity of spinning tubes from 1.1ml to 85ml, all with aerosol tight caps. Fixed angle or swing out, swing bucket rotor options for these centrifuges, as a refrigerated and now heated model is sold. In fact most of our centrifuges are available as refrigerated. A 30 x 15ml Falcon Tube rotor is an option, along with 90ml square buckets for even greater capacity.

    Eppendorf 5810R Multipurpose Centrifuge The larger general purpose centrifuge should feature the options of refrigeration, fast cool function, at set RPM, automatic rotor recognition, motorized lid latch, imbalance cut-off, fulfill all current safety standards; speed, rcf and radius correction values correction values can be entered by the user; values can be changes during centrifugation; temperature range of -9 to 40 degrees centigrade [on refrigerated models]; stand-by refrigeration; speed can be set from 200 rpm to maximum speed in increments of 10rpm; selectable program memory for up to 34 individual user programs; 10 acceleration and 10 braking ramps for sensitive samples. Tube, deepwell plates, microplate, microtitre and PCR plate adaptors / buckets are available as well as tube adaptors for tubes between the sizes of 1.5ml and 400ml flasks. Now new buckets also allow our range of centrifuges to spin microscope slides and cell culture flasks! Fixed angle and swing out rotors, fully autoclavable are available too.

    All POCD Scientificsupplied Eppendorf Centrifuges come with a two year warranty, fully supported by POCDS's factory-trained service department.


    A brief history of the Centrifuge

    an antique centrifuge

    Centrifuges similar to our bench top centrifuges have been in use since the mid-1800’s. These centrifuges were capable of speed of about 3000 rpm. The early ones were hand driven; after 1912, the centrifuges were electrically driven. First applications were non-biological, such as separation of milk and collection of precipitates.

    Miescher (1872) attempted to separate macromolecules – credited with the discovery of nucleic acids.

    The development of the ultracentrifuge is generally credited to Svedberg who worked in the 1920’s and 1930’s. Svedberg coined the term “ultracentrifuge.” Svedberg was a colloid chemist - he studied the structure of proteins (at that time all proteins were considered to be colloids). His group used the ultracentrifuges to determine the MW and subunit structure of hemoglobin, studies which changed the ideas concerning the structure of proteins. Svedberg’s group developed a number of ultracentrifuges. Some of the early models were capable of reaching 900,000xg, but the rotors were very small. For routine work they used larger rotors which could reach 260,000xg.

    The first commercial ultracentrifuge was produced in 1940 by SPINCO.

    Types of Centrifuges

    • Desk top clinical centrifuges. The maximum speed of most desk model centrifuges is below 3000 rpm and all of them operate at ambient temperature.
    • Highspeed centrifuges are those operating up to speeds of 20,000 to 25,000 rpm.
    • The ultracentrifuge has the ability to attain centrifugal forces in excess of 5000xg (75,000, r=8cm). The development of the ultracentrifuge permitted the fractionation of subcellular organelles previously observed only in electron micrograph; this in turn permitted assay of their enzymatic constituents, providing insights into structure-function relationships. The contemporary ultracentrifuges consist of four principal parts: drive and speed control, temperature control, vacuum system and rotors.
      • Drive and speed control. The drive shaft itself is only about 3/16 in in diameter. The samll diameter of the shaft allows it to flex during rotation, thus accommodating a small degree of rotor imbala ce without vibration or spindle damage. An overspeed system was developed to prevent operation of a rotor above its maximum rated speed. Such operation results in the rotor being torn apart or exploding. It is for this reason that the rotor chamber is always enclosed in heavy armor plate capable of containing any such explosion. The over speed system consists of (1) a ring of alternating reflecting and non-reflecting surfaces attached to the bottom of the rotor., (2) a small but intense point source of light, and (3) a photocell. The passing of reflecting and nonreflecting surfaces throught the light beam as a consequence of rotor rotation chaps the light and establishes a pulsing signal in the photocell output circuitry. The frequency of this signal, which is a function of the speed of rotation, is compared to a standard reference signal. If the rotor-generated signal frequency surpasses that of the reference, the instrument is automatically shut down.
      • Temperature control in highspeed instruments involves placing a thermocouple in the rotor chamber, and monitoring only the rotor chamber temperature. In the case of an ultracentrifuge and infrared radiometric sensor placed beneath the rotor continuously monitors the rotor temperature directly, ensuring more accurate and responsive temperature control.
      • Vacuum System. At speeds below 15,000 to 20,000 rpm only small amounts of heat are generated by friction between air and the spinning rotor. At greater speeds, however, air friction is significant and becomes severe above 40,000 rpm. To eliminate this source of heating, the rotor chamber is sealed and evacuated by two pumping systems operating in tandem. The first system is a mechanical vacuum pump similar to those in normal laboratory use, which can establish a vacuum down to 100 to 50u. Once the pressure in the chamber has decreased below 250u, a water-cooled diffusion pump is also brought into operation. Using both pumps it is possible to attain and hold vacuums of 1 to 2 u. As would be expected, temperature control is significantly improved when the rotor chamber is evacuated.
      • Rotors - A large variety of rotors are available for use in modern ultracentrifuges, and fall into two classes: angle and swinging bucket. Both types are constructed from either aluminum alloys for low to moderate speeds or titanium for highspeed operation. Angle rotors consist of a solid piece of metal with 6 to 12 holes machined at an angle between 20o and 45 o. These rotors are most often used for applications involving total sedimentation or “pelleting” of a constituent. Swinging bucket rotors consists of a rotor from which hang three to six free moving buckets. These buckets hang vertically when the rotor is at rest and swing 90 o to a horizontal position, under the influence of centrifugal force, as the rotor attains a speed of 200 to 800 rpm. This type of rotor was designed principally for incomplete sedimentation of a sample through some sort of gradient. In this position material sedimented to different areas of the tube appearing as bands running across the tube rather than at an angle as in an angle rotor.

    Centrifuges have sometimes been at the forefront of technological innovation

    Centrifuges have sometimes been at the forefront of technological innovation


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    www.pocdscientific.com.au/centrifuges_centrifugation.php       updated April 4th,2013
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