| Are all parts that I need for the connection of the FemtoJet/FemtoJet express to the InjectMan NI 2 included in the standard accessories or are additional parts required? |
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All necessary cables and tubes are included in the standard accessories. However, the adapter for the special microscope must be ordered separately.
| How do you remove pieces of broken glass capillaries from the capillary holder? |
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For this purpose we offer a Service kit (5176 195.004) that contains an appropriate tool. Alternatively you can also use a thin metal wire.
| Can FemtoJet/FemtoJet express be controlled externally, e.g. by a TTL connection? |
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Yes. The device can be controlled externally via an RS 232 interface.
| The pressure tube connected with the universal capillary holder 5176 190.002 is missing. What do I have to order? |
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"According to the injectors in use you have to order the following tubes:
Connection to CellTram Air/Oil/vario: 5176 114.004
Connection to Transjector/FemtoJet/FemtoJet express: 5246 164.004"
| What is the compensation pressure pc? |
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The compensation pressure pc ensures that no culture medium flows into the capillary during microinjection experiments. Capillary forces would make liquid flow out of the cell culture dish into the injection capillary and thus dilute the injection material. To prevent this, a permanent compensation pressure pc is set. This should be selected so that there is a permanent slight flow-out of liquid from the injection capillary. The individual pressure level can be determined in a preliminary test.
| What is the outer diameter of the universal capillary holder? |
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The outer diameter is 4 mm.
| I want to use my own capillaries with Eppendorf microinjectors. Which capillary grip would fit my tips? |
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"All Eppendorf microinjectors (Transjector, FemtoJet/FemtoJet express and CellTram) are equipped with the Universal Capillary Holder. Different grip heads can be interchanged for the use of capillaries with different outer diameters. Use the table below to determine which grip head suits your needs.
Description
Capillary grip 0: fits microcapillaries with an outer diameter of 1.0 to 1.1 mm, order no. 5176 210.003
Capillary grip 1: fits microcapillaries with an outer diameter of 1.2 to 1.3 mm, order no. 5176 212.006
Capillary grip 2: fits microcapillaries with an outer diameter of 1.4 to 1.5 mm, order no. 5176 214.009
Capillary grip 3: fits microcapillaries with an outer diameter of 0.7 to 0.9 mm, order no. 5176 207.002 "
| Which grip head is included in the delivery package of the FemtoJet/FemtoJet express? |
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Grip head number 0. It can be used for capillaries with an outer diameter of 1,0-1,1 mm.
| "How can the different grip heads be distinguished?" |
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The grip heads have different numbers of notches. The grip head 0 has no notch at all; the grip head 3 has, for example, three notches