Small, fast and flexible for all the latest PCR applications
We sell, supply, service and carry spare parts for these products.
All equipment servicing to manufacturer’s specs by Eppendorf factory certified technicians.
Calibration, repair, service, spare parts, Compliance Covering - IQ/OQ/PQ, calibration and validations.
On-site repairs are available for customers in Sydney, Melbourne and Brisbane.
For other areas/States, we offer a workshop service or spare parts.
Eppendorf Mastercycler ep Realplex ~ overview
Small, fast and flexible-the Mastercycler ep realplex real-time PCR system meets all the latest requirements of quantitative PCR applications. Extremely fast temperature ramping, short detection times and intuitive assay programming translate to huge time-savings so that more experiments can completed per day-always with accurate and reliable results. Itâ€˛s flexibility is beyond compare-this completely open system allows you to use tubes, plates and reagents of your choice. No other real-time cycler on the market today offers such freedom.
New! Modified Slip Cover (light transmissive cover for optical openings in heated platen assembly, US patent pending).
Mastercycler ep realplex is compact in size, so it can fit in virtually any lab, no matter how limited the benchspace. Itâ€˛s modular and therefore makes upgrading easy, for example: realplex allows you to upgrade your Mastercycler ep gradient S to a real-time PCR cycler at any time; the realplex2 module can be easily replaced by the realplex4 module if/when multiplexing becomes essential.
Innovative Eppendorf Thermal Sample Protection technology prevents premature heating of the samples, which significantly reduces the amplification of nonspecific PCR products during this heating phase. And a solid construction with minimal moving parts makes this a quiet, dependable unit that promotes consistently high sensitivity in your real-time PCR experiments.
Lifetime Warranty on LEDs in the MastercyclerÂ® ep realplex
The lifetime warranty on LEDs in the Mastercycler ep realplex guarantees you an excitation source that will hold up to the specifications for a lifetime! No periodic replacing, no relevant degradation between experiments â€“ just the same quality and quantity of light again and again and again â€¦
The 96 LEDs in the Mastercycler ep realplex are build to hold! Thus, Eppendorf will replace the array of LEDs free of charge if the LEDs do not perform as specifi ed â€“ anytime. This way, you do not have to worry about the excitation light of your real-time PCR instrument and can focus on your experiments.
Eppendorf Mastercycler ep Realplex ~ features
Quality in all forms.
The new optical system of the Mastercycler ep realplex
offers a new proprietary particle protection technology*
and very sensitve photomultipliers of the latest generation.
All thermomodules have special surface-treated thermal blocks in the 96-well format, and they are equipped with the SteadySlopeÂ® gradient technology. Choose from two thermomodules, depending on your requirements. The â€śstandardâ€ť variant is fi tted with a specially fi nished 96-well aluminum block, while the â€śhigh-speedâ€ť variant has a 96-well silver block with
Impulse PCR and extremely fast heating and cooling rates. Regardless of the thermomodule selected, reproducible results can be attributed to the highest accuracy and uniformity of Mastercycler ep thermal cycling.
Both thermomodules can be combined with two different optical modules, realplex2 and realplex4, which permit the use of nearly all fluorescent dyes used in real-time PCR. The realplex2 module has 2 fluorescence filters and one photomultiplier (PMT), while the realplex4 module incorporates 4 filters and two PMTs for 4-fold multiplexing assays.
*Patent pending Automated Real-time PCR
Mastercycler ep realplex & epMotion 5070 automated pipetting - Automated real time PCR for everyone!
Real time PCR results depend pivotal on the quality of pipetting. qPCR expert S.A. Bustin demonstrated the operators influence when manually pipetting real time PCR. In his experiments the replicate Cts obtained by three individuals vaired between 8Â·7Ă—105 and 2Â·7Ă—103 copy numbers/Âµg total RNA.
(Journal of Molecular Endocrinology 29/2002). He concludes these "results provide a convincing argument for the use of robots when reproducibility and inter-laboratory comparability are the main concerns".
Real quantitative PCR needs automated pipetting
Automated epMotion pipetting can show a significant reproducibility advantage, when compared to manual set-ups. With a pipetting range down to just 1Âµl, its unmatched accuracy, reproducibility and easy to use philosophy mean the epMotion is the unique pipetting system for qPCR.
epMotion automated qPCR set-up guarantee highly consistent and reproducible results within and across experiments. Reactions can be performed in lower volumes and with fewer replicates, thereby reducing reagent cost significantly.
Real time PCR needs real speed
The Mastercycler ep realplex is the perfect partner for automated real time PCR. Powered by the highly accurate and uniform Mastercycler ep thermal engine, the realplex defines a new dimension of speed for automated real time PCR. Utilising the high speed silver block and impulse PCR technology, amplification can be performed in less than 30 minutes.
Packaged in a compact and robust design matched with a peace of mind real time-PCR licence ensure the Mastercycler ep realplex is a real marvel in space and time.
Mastercycler ep realplex
Real-time PCR in less than 30 minutes
Highly sensitive optical system
epMotion 5070 automated pipetting system
Easy operation via control panel or PC
Preinstalled, validated applications and labware
High-precision pipetting from 1 Âµl to 1,000 Âµl
The real team
Real intuitive software and affordable packages: epMotion 5070 and Mastercycler ep realplex make automation of real time PCR available to everyone. It was never so easy to become state of the art.
Application note 119: Automated Quantitave PCR Set-up using the epMotion 5070 workstation
epTips Starter Pack (enough for at least 10 method runs)
1 x 50 Âµl Filter, cat.-no. 0030 003.950
The fluorescent dyes chosen for each experiment are excited by an array of 96 individual LEDs, which have a substantially longer lifespan in contrast to halogen lamps.
The fluorescent dyes chosen for each experiment are excited by an array of 96 individual LEDs, which have a substantially longer lifespan in contrast to halogen lamps. The result is a real-time PCR system that is less susceptible, minimizing equipment downtime and the frequency of maintenance and associated delays to your experiments.
The LEDs generate a blue light at a wavelength of ~470 nm, which can excite nearly all fluorophores used in realtime PCR (including SYBRÂ®Green, FAM, VIC, TET, HEX, ROX, JOE, TAMRA). Emitted fluorescence is focused through an array of lenses and is passed to the optical detection unit through 96 individual optical fibers.
Immediately upstream of the detectors, two high-quality emission filters with bandwidths of 10 nm to 30 nm are contained in a filter rotor.
New type channel photo-multipliers (CPM) serve as the detectors, which in contrast to conventional photomultiplier tubes, are far less sensitive to magnetic field interference.
Mastercycler ep realplex light path
Light emission from 96x LED array (470 nm)
a/b/c focusing with 96x lens arrays
Semipermeable beam splitter
Light emission from excited fluorescent dyes in the reaction mixture (see representation in the graphic)
Merging of the rays through a "96-in-1" fiberglass strand
Focusing; passage through additional beam splitters
Filter wheel with interference filters
Forwarding of the light ray to the photo multiplier
Over a linear dynamic range the Mastercycler ep realplex detects up to 9 levels of magnitude.
Mastercycler ep realplex provides an optional melting curve analysis module. Thus, specific PCR products can be differentiated from nonspecific products such as primer-dimers. Melting curve analysis is also a key tool for the genotyping of SNPs and for detecting the smallest deletions.
Programmed for success
With a total of six different evaluation modules realplex software offers maximum flexibility for data processing. Data results can also be imported into MicrosoftÂ® Excel at any time to comply with established analysis and documentation procedures in your laboratory.
The complete realplex software package has been designed with "user-friendly" in mind: the intuitive user interface ensures a simple, fast PCR and assay set-up as well as simple transfer of PCR protocols that have been previously established on other real-time PCR systems.
The clear and comprehensive organization of multiple viewing windows as well as intuitive options for selection within each view enable easy and fast plate setup when creating assays.
of this analysis module generates allele-discrimination results. Data previously generated in melting curve analyses, endpoint measurements or Ct-value determinations serves as the basis.
This software module makes it possible to observe the generated raw data in real time, which means that you can immediately evaluate your real-time PCR.
The analysis module option for endpoint measurements instructs the Mastercycler ep realplex to serve as a "plate fluorometer".
Quantification / relative Quantification
The analysis module performs calculations from raw data for a variety of assays, including absolute quantification of DNA or relative quantification of gene expression based on the ΔΔCt method.
Critical threshold values can be defined to enable the differentiation between positive and negative samples (e.g., for the detection of pathogens).
Real-time PCR Licence
It is and always has been Eppendorfs policy to respect intellectual property of other parties. To make a licensed real-time PCR solution available to our customers, we have acquired a license for our real-time PCR products, including the Mastercycler ep realplex. We believe that only a licensed instrument is giving our customers the necessary safety and reliability to implement such a technology in the laboratory routine. If you want to know more about our real-time PCR license, please contact POCD Scientific.
Practice of the patented polymerase chain reaction (PCR) process requires a license. The Eppendorf [or appropriate trademark] Thermal Cycler is an Authorized Thermal Cycler and may be used with PCR licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents. This is a Licensed Real-Time Thermal Cycler under Applera's United States Patent No. 6,814,934 and corresponding claims in non-U.S. counterparts thereof, for use in research and for all other applied fields except human in vitro diagnostics. No right is conveyed expressly, by implication or by estoppel under any other patent claim.
*New! Modified Slip Cover (light transmissive cover for optical openings in heated platen assembly, US patent pending).
96 x 0.2 ml PCR tubes or one 96 PCR plate
(unskirted, semi-skirted, sikrted-as per SBS standard)
Temperature control range of block
Degree range of gradient, max.
1-20°C (thermomodule Mastercycler ep)
1-24°C (thermomodule Mastercycler ep S)
Temperature control range of gradient
Temperature of lid
Heating speed *
approx.4°C/s (thermomodule Mastercycler ep)
approx.6°C/s (thermomodule Mastercycler ep S)
Cooling speed *
approx.3°C/s (thermomodule Mastercycler ep)
approx.4.5°C/s (thermomodule Mastercycler ep S)
Dimensions /(W x D x H)
26cm x 41cm x 39.6cm
Weight of thermomodule
Weight of detection module
200-240V, 50 Hz - 60 Hz
* Measured at block
**New! Modified Slip Cover (light transmissive cover for optical openings in heated platen assembly, US patent pending).
Eppendorf Mastercycler ep Realplex ~ ordering
We have endeavoured to provide Australian catalogue numbers; however it would be prudent to verify any catalogue numbers taken straight from our site with POCD Scientific before using them to place an order.
Mastercycler® ep realplex :
Mastercycler® ep realplex : 2, with aluminium block and two emission filters
Mastercycler® ep realplex2 S, with silver block and two emission filters
Mastercycler® ep realplex4, with aluminium block and four emission filters
Mastercycler® ep realplex4 S, with silver block and four emission filters
Optical detection module to upgrade Mastercycler ep gradient / S realplex2 & realplex4
contact POCD Scientific
New! Modified Slip Cover (light transmissive cover for optical openings in heated platen assembly, US patent pending).
Covered by US Patent Nos. 6,210,958; 6,767,512 and 7,074,367
Can I perform relative quantification with the Mastercycler ep realplex?
Yes, a data analysis module for relative quantification (DDCt method) is included in the software.
What are the key benefits of the Mastercycler ep realplex?
The Mastercycler ep realplex brings advanced real-time PCR technology to the benchtop with its highly intuitive operation and analysis software, and its extremely robust, compact design. The realplex combines the advanced, high speed gradient thermal block design of the Mastercycler ep thermal cycler family with highly sensitive optical detection technology of wide dynamic range so you can analyze more samples with greater flexibility in a single plate.
Can I run my established protocols on the Mastercycler ep realplex?
Established protocols can easily be adapted to the Mastercycler ep realplex. The temperature ramp rates can be fine-tuned to mimic the characteristics of other qPCR platforms. Furthermore, the realplexâ€™s gradient capabilities enabling you to test a range of temperatures across 12 columns of the plate.
How fast is the Mastercycler ep realplex silver or aluminium block?