POCD Scientific ~ Eppendorf’s Australian distributor for 0.1, 0.2 ml and 0.5 ml PCR Tubes ~ overview

These are POCDS Time Critical Products - we are seriously committed to having these products in stock 24/7/365 for immediate shipping. If we are unable to immediately dispatch any of these items to you upon receiving your order we will airfreight the item from Germany at our expense to minimise the delay in getting the item to you.

Original Eppendorf PCR tubes are manufactured to the highest Eppendorf quality standards. These thin-walled polypropylene tubes ensure efficient and homogeneous heat transfer in the sample thanks to the even wall thickness and smooth wall surface. Heat-sealed bags enclosed in boxes sealed with a PCR Clean sticker ensure the highest degree of purity.

A frosted labeling area on the tube allows easy sample tracking.

NEW: Larger lid of 0.5 mL PCR Tubes

The new 0.5 mL PCR Tubes have a larger lid. This new lid offers a larger labelling surface but it may also lead to a different fit in your cycler or used adapter.

NEW: PCR Tube Strips 0.1 mL

POCD Scientific ~ Eppendorf’s Australian distributor for 0.1, 0.2 ml and 0.5 ml PCR Tubes ~ features

• Frosted area for easy labeling
• High transparency
• Certified free from human DNA, DNase, RNase and PCR inhibitor
• Tight sealing
• Easy to open
• For use with all thermal cyclers with 0.5 ml block format

• Contamination shield on hinged lid
• Defined lid position due to special hinge
• High transparency even on the tube base
• Etched lid for additional writing surface
• Tight sealing
• Easy to open
• For use with all thermal cyclers with 0.2 ml block format
• Also availabe in 8-tube strip format
• Certified free from human DNA, DNase, RNase and PCR inhibitor

• 0 8 reaction tubes in strip-format - ideal for small sample volumes
• Easily adaptable for automation
• Sealable using flat or domed strip-lids
• Certified free from human DNA, DNase, RNase and PCR inhibitors

• The work tray is placed into a frame to form a rack for 96 0.2 ml PCR tubes
• Easy to use: With the work tray, all tubes are loaded into the thermoblock simultaneously
• Both parts are polycarbonate and can be autoclaved (121°C, 20 min.)
• Rack for 0.2 ml PCR tube consisting of work tray and frame

Some background on PCR and its origins

The polymerase chain reaction (PCR) is a molecular biology technique which amplifies a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of an individual DNA sequence.

Developed in 1983 by Kary Mullis, PCR has become a common and often indispensable technique used in medical and biological research labs for a variety of applications.

These include DNA cloning for sequencing, DNA-based phylogeny, or functional analysis of genes; the diagnosis of hereditary diseases; the identification of genetic fingerprints (used in forensic sciences and paternity testing); and the detection and diagnosis of infectious diseases. In 1993, Mullis was awarded the Nobel Prize in Chemistry for his work on PCR.

The method relies on thermal cycling, consisting of cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA. Primers (short DNA fragments) containing sequences complementary to the target region along with a DNA polymerase (after which the method is named) are key components to enable selective and repeated amplification. As PCR progresses, the DNA generated is itself used as a template for replication, setting in motion a chain reaction in which the DNA template is exponentially amplified. PCR can be extensively modified to perform a wide array of genetic manipulations.

Almost all PCR applications employ a heat-stable DNA polymerase, such as Taq polymerase, an enzyme originally isolated from the bacterium Thermus aquaticus. This DNA polymerase enzymatically assembles a new DNA strand from DNA building blocks, the nucleotides, by using single-stranded DNA as a template and DNA oligonucleotides (also called DNA primers), which are required for initiation of DNA synthesis.

The vast majority of PCR methods use thermal cycling, i.e., alternately heating and cooling the PCR sample to a defined series of temperature steps. These thermal cycling steps are necessary first to physically separate the two strands in a DNA double helix at a high temperature in a process called DNA melting. At a lower temperature, each strand is then used as the template in DNA synthesis by the DNA polymerase to selectively amplify the target DNA.

The selectivity of PCR results from the use of primers that are complementary to the DNA region targeted for amplification under specific thermal cycling conditions.