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EPPENDORF ThermoMixer® C
Improve your assay results by dry incubation and mixing of your samples simultaneously: The Eppendorf ThermoMixer C is for heating, cooling, and mixing almost any of your lab vessels. Reliable prevention of condensate formation in small vessels can be achieved with the heated lid Eppendorf ThermoTop (optional).
The Eppendorf ThermoMixer C combines unique mixing performance with excellent temperature control to consistently guarantee complete, dependable and reproducible test results. Improve your assay results by mixing and incubating samples at the same time.
The Eppendorf ThermoMixer® C convinces users with its quick and easy-to-exchange SmartBlocks for all common lab vessels, new and improved program functions, and high level of stability for every mixing frequency. There is no limit to the variety of applications waiting for you!
Extraction of DNA fragments from agarose gels
Enzymatic reactions (e.g., DNA restriction digestion, Proteinase K digestion and ligation)
Transformation of bacteria strains and plasmids
Denaturation of DNA, RNA and proteins
Labeling of DNA, RNA and proteins
Bacterial growth in microreaction vessels, conical tubes and deepwell plates
Lysis reactions at 100 °C
Mixing of PCR setups
Excellent mixing results:
Increased mixing frequency up to 3,000 rpm (depending on the SmartBlock used)
Unique 2DMix-Control technology mixes liquids in a controlled, circular movement for fast and efficient mixing in tubes and plates
Anti-spill technology reliably prevents lid wetting and cross-contamination
Outstanding temperature management:
Unique Eppendorf ThermoTopÂ prevents condensation and improves temperature homogeneity for improved assay performance
Individually sensor-controlled Eppendorf SmartBlocksâ„¢ provide maximum temperature accuracy and block homogeneity
Dry incubation technology reduces contamination risk and increases lab hygiene
Ergonomics and simplicity:
Eppendorf QuickReleaseâ„¢ technology for quick exchange of Eppendorf SmartBlocksâ„¢
Low noise level for stress-free work
Easy to operate using predefined program keys or temperature keys
More Features of the Eppendorf ThermoMixer C:
Heating, mixing and cooling in all common vessel and plate formats from 5 ÂµL to 50 mL
Maximum flexiblilty due to large selection of exchangeable SmartBlocks
Versatile program functions
Ergonomic design and optimized features according to the Eppendorf PhysioCare Concept
2DMix-Control technology describes the ability of the MixMate, ThermoMixer C and ThermoMixers F to mix small sample volumes in various plate and tube formats quickly and in a controlled manner. To achieve this, the interplay of all crucial factors for efficient and controlled mixing, have been optimized. And these are: <br />- Generation of sufficient mixing energy: due to an orbital mixing movement at a high mixing speed and a relatively large mixing radius (1.5 mm) the devices generate enough mixing energy (analogous to g-force in centrifuges) to mix even the smallest volumes efficiently.<br />- Minimized loss of mixing energy: special attention was paid to reducing inherent vibration. Smooth operation combined with reliable stability ensures that all the mixing energy is available for mixing and not wasted through transmission of vibrations to the device or its surroundings.<br />- Controlled transmission of energy from the device to the sample: Minimizing vertical movements enable a plane, 2-dimensional (2D) mixing stroke. This, in conjunction with the reliable plate and tube holders and SmartBlocks, prevents uncontrolled chaotic movements and allows all formats to be held securely, even at high mixing frequencies.
Do not use corrosive cleaning agents, aggressive solvents or abrasive polishes. Do not use any aggressive chemicals on the device or its accessories, such as strong and weak bases, strong acids, acetone, formaldehyde, halogenated hydrocarbons or phenol. If the device becomes contaminated with aggressive chemicals, clean it immediately with a mild cleaning agent. Use Lint-free cloth, mild, soap-based lab cleaner and dist. Water. Please refer to your operating manual chapter Maintenance.
The best possible temperature accuracy can be guaranteed by individually adjusting a SmartBlock. Optimized temperature control for each block type keeps temperature overshoots and deviations caused by external interferences to a minimum. To guarantee optimal performance over the entire period of use, Eppendorf offers a preventive maintenance program and certification services: your Eppendorf ThermoMixers, ThermoStat C as well as the SmartBlocks are regularly cleaned, checked and adjusted. Additional information is available at: https://www.eppendorf.com/epservices<br /> . Customers can also verify the temperature accuracy by themself. Eppendorf provides for that purpose the Temperature Verification System – Single Well for Eppendorf Mastercyclers (0056 000.003) in combination with the 384 well Sensor (0056 002.006).
Yes, one can use the devices in a cold room but some safety hints should be attended: Condensate can form in the device after it has been moved from a cool environment to a warmer environment. Wait at least 3 hours after positioning the device. Plug the device only after this time has elapsed. In case the device is positioned continually in a cold room and was switched off for a longer time – over night for instance - switch it on for at least 1 h before starting a mixing process.
The ThermoMixers F possess a fix installed SmartBlock and can only be used with the appropriate microtest tubes or plates, such as MTP’s, DWP’s or skirted plates complying the SBS standard. For using PCR plates our SmartBlocks PCR 96 and PCR 384 in combination with ThermoMixer C is the proper choice.
Please check if a compatible SmartBlock has been attached and the ThermoTop has been vertically placed from above. The centering pins behind the heating/cooling plate have to fit perfectly into the recesses of the ThermoTop. Place the ThermoTop once again upon the device. In case the device shows still the error message, please contact the technical service.
SmartBlock plates and ThermoMixer FP (with the fixed SmartBlock plates) are dedicated for using MTP’s, DWP’s and also other skirted plates which meet the SBS standard. For optimal usage of PCR plates the SmartBlock PCR 96 and PCR 384 in combination with ThermoMixer C or ThermoStat C is the proper choice. These SmartBlocks possess conical bores which surround the conical wells of PCR plates properly for a perfect temperature transition.
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