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The Multipette® E3x offers additional high-end applications, such as titration and aspirating of supernatants or sequential dilutions. In combination with the Combitips advanced®, it is an essential lab tool for all kind of liquid handling.
The Multipette E3/E3x in combination with the Combitips advanced® creates a versatile dispensing system that functions according to the positive displacement principle and is therefore capable of pipetting any liquid while maintaining the highest standards for accurate results. The motor driven dispenser gives you full volume control, improved accuracy and reproducibility while reducing physical strain during use. The repetitive dispensing modes contribute to reducing fatigue by decreasing the number of movements for the user; filling the tip once allows dispensing up to 100 times. The system is an essential lab tool ideal for all liquid handling applications, accommodating more than 5,000 different dispensing volumes between 1 μL to 50 mL.
Combitips advanced tips easily attach to the Multipette; their built-in sensor recognises the size of the tip, and the volume appears automatically in the display—eliminating time-consuming volume calculations and incorrect dispensing volumes. The nine sizes of Combitips advanced allow a wider dispensing range of up to 112 different volumes and longer series dispensing (e.g., 100 x 250 µL) without stopping for a refill. Always contamination-free!
Combitips advanced tips function according to the positive-displacement principle, which ensures that the correct volume is dispensed regardless of the density or viscosity of the liquid. The positive displacement system provides safer dispensing of solutions that contain radioactive and/or toxic substances, as well as solutions that have a high vapour pressure, because no aerosols are formed. Combitips are easily replaced—eject with the push of a button— and dispose after use, always contamination-free.
The Multipette/Combitips system is a sophisticated solution for repetitive liquid handling applications. These contamination-free manual and electronic systems are designed with your productivity and safety in mind—especially when working with hazardous, volatile or viscous liquids.
The Multipette/Repeater E3 has three functions: dispensing, automatic dispensing and pipetting. The Multipette/Repeater E3x has four additional functions: sequential dispensing, aspiration, aspiration/dispensing, and titration. Both models use Combitips advanced.
The Multipette/Repeater E3 and E3x are stable to UV light. In the event of prolonged irradiation with UV light, material discolorations may occur, but this does not affect the proper function of the Multipette/Repeater.
To calibrate Multipettes/Repeaters in accordance with EN ISO 8655, three different volumes with ten measured values each have to be checked. These three volumes are the nominal volume (= the maximum volume to be dispensed), 50% of the nominal volume and 10% of the nominal volume of the Combitips advanced used. The checking procedure, the test conditions, and the limits in accordance with EN ISO 8655 are described in our "Eppendorf SOP for checking pipettes". The SOP is available on our web page.
Mode A/D allows the aspiration of an unknown volume and its subsequent dispensing in equal parts. The usage of the A/D mode is advantageous for several applications in protein biochemistry, molecular biology, cell biology, and microbiology. Detailed information on different possible applications is given in White Paper No. 25.
The measuring values of the analysis scales are multiplied by the correction factor Z (see SOP pipettes). This takes into account the density of the water at the relevant temperature and the relevant air.
ISO 8655 addresses the needs of suppliers, test houses, and users and specifies the general requirements for piston-operated liquid handling devices. It is structured in seven chapters:<br />ISO 8655-1: Terminology, general requirements, and user recommendations<br />ISO 8655-2: Piston pipettes<br />ISO 8655-3: Piston burettes<br />ISO 8655-4: Dilutors<br />ISO 8655-5: Dispensers<br />ISO 8655-6: Gravimetric method for the determination of measurement error<br />ISO 8655-7: Non-gravimetric methods for assessment of equipment performance (titration, photometry)
The systematic error describes the deviation of the dispensed volume from the nominal volume or from the selected volume of the piston stroke device, and is determined by establishing the mean value of (usually) 10 measurements. The random error describes the mean variation of the dispensed volumes from the mean value of the dispensed volumes, and is determined by calculating the repeat standard deviation of (usually) 10 measurements.
The Z factor is included in EN ISO 8655-6 and was incorporated into our SOP. As we understand it, it was incorporated in the standard by the Physikalisch Technische Bundesanstalt in Braunschweig.<br />The Z factor describes the density of water at a particular temperature using a 5th degree polynomial. Furthermore, the density of water is corrected by the density of air (derived from air pressure) and a fixed correction value for balances. These corrections are the key reasons for the deviation from density values in various books of tables.
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