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Eppendorf Xplorer® / Xplorer® plus
With the Eppendorf Xplorer®, your work achieves a new level of simplicity, precision and reproducibility. No more delays due to complicated programming or inflexible processes.
You know a product is the undisputed best of its class when it continues to win professional accolades such as these
2010: Red Dot (“best of the best”) trendsetting design award
2010: Golden Pin Design Award for design excellence
2011: iF Product Design Award for innovative product design.
2011: Scientist's Choice Award “Best New General Lab Product of 2010”
You also know the results you obtain when using the best product are as good as the product: simply the best. And we haven't even mentioned the fulfilling feeling of using a prestigious award-winner: its like driving a Rolls-Royce Ghost Passenger Car – another winner of the 2010 Red Dot award!
People who give 100 % every day deserve the best tool and the best equipment. You work on demanding problems and important decisions depend on the results of your work. Your standard is extremely high and your performance is professional.
The new Eppendorf Xplorer® automatic electronic pipette was specially designed for high professional standards to provide optimal support for you in your work, with a new intuitive operating concept and design based on the proven Eppendorf PhysioCare Concept®.
These features set new standards for simplicity, precision and reproducibility, which means no more delays due to complicated programming or inflexible processes. Instead, you get precisely adjustable parameters, maximum reproducible results, fatigue-free work and consistent, full control over the pipetting processes
Features
Work Intuitively
Multifunctional rocker: 'Up is up and down is down' principle
Easy selection of all functions with the practical selection dial
Operate in the language of your choice
Clearly arranged color display
No sub-menues
Help function
Innovative rocker: When the tip is ejected, the piston automatically returns to zero position
PhysioCare Concept®
Reduced weight
Improved ergonomics
Perfect balance
Optimally angled pipette head
Improved view & high resolution display
Spring-loaded tip cone provides maximum tightness
Reliably reproducible results
Tips always rest in the same position
Volume & speed precisely und reproducibly adjustable
Adjustable to your work environment and requirements
Additional Features of the Eppendorf Xplorer plus:
On/off switch: Switches the pipette off during longer periods of inactivity
Additional applications: Aspirating, diluting, sequential dispensing and reverse pipetting
Programming: One-time programming of sequences for easier handling of complex processes
Individual fixed volume setting: Desired volume can be saved
Password protection: Protects programming and adjustment
Password protection: Protects programming and adjustment
Individual speed setting: Sets parameters to your individual working speed
Display of service intervals: Pipette maintenance reminder function, based on time or frequency of use as desired
Reverse pipetting means uptake of liquid including the blow-out volume and dispensing of liquid without the blow-out volume. Reverse pipetting is recommended to reduce the error with viscous or foaming liquids or when dispensing very small sample volumes. The excess solution aspirated remains in the tip and is discarded.
When pipetting viscous liquids, we recommend positive displacement instruments like Biomaster, Multipette/Repeater, and Varipette or an air cushion pipette (Research plus, Reference 2) in combination with the reverse pipetting technique (see Userguide 20 and 21 for details).
When pipetting chloroform, we recommend positive displacement instruments like Biomaster, Multipette/Repeater, and Varipette or an air cushion pipette (Research plus, Reference 2) in combination with the reverse pipetting technique (see Userguide 20 and 21 for details).
Diethyl ether has such a high vapor pressure that it would leak out of the tip immediately when an air cushion pipette is used. For this reason, it can only be pipetted using a positive displacement pipette, such as Biomaster, Multipette/Repeater, and Varipette.
A 99 % solution of n-amyl alcohol is a highly viscous solution. We recommend working with an instrument that operates according to the positive displacement principle like Multipette/Repeater, Varipette, or Biomaster.
It is best to use a positive displacement device like the Multipette/Repeater. Positive displacement devices are most suitable for precise and drip-free dispensing of liquids with high vapor pressure. As the liquid is enclosed in a tip with integrated piston, the solvent vapor does not come into direct contact with the instrument.
Both dichloromethane and n-hexanol have a very high vapor pressure. For that reason, positive displacement instruments like the Biomaster and the Multipette/Repeater should be used.
No, we cannot guarantee this. Of course we work carefully and cleanly in production, but no special measures are taken to prevent the infiltration of nucleic acids. To protect your pipette from contamination, we recommend using positive displacement pipettes, for example, our Biomaster or filtertips (ep Dualfilter T.I.P.S. or ep Dualfilter T.I.P.S. SealMax).
Following contamination of pipettes with radioactive liquids, we recommend placing the contaminated parts in a complex-forming liquid or special cleaning solution. Then rinse very thoroughly with distilled water and dry. Grease piston lightly afterwards.
Exactly in the same way as liquids at room temperature, however, please note that solutions may behave differently. In addition, the pipetting accuracy / precision may be outside the permissible error limits. In order to achieve maximum accuracy / precision, all components should have the same temperature: pipette, tips, liquid, and the surrounding air.
Temperature effects are minimised in our pipettes by their design. We use a special polypropylene which satisfies the special requirements on a pipette handle.
This belongs to the permissible errors of your specific application. In general an adjustment of air cushion pipettes, in other words altering the stroke of the pipette, is required for solutions of a density größer 1.2 mg/µL. <br />Please be aware that variable manual pipettes turn into "fixed-volume pipettes" when being adjusted: They can only be adjusted to a specific liquid to one volume and not across the entire dispensing range.<br />Eppendorf manual pipettes have a user adjustment for temporary adjustment purposes. This user adjustment can easily be set back to manufacturer settings without the need for recalibration. For the correct procedure and examples for settings of user adjustments for different liquids please see the operating instructions and CD enclosed to your pipette.
The measuring values of the analysis scales are multiplied by the correction factor Z (see SOP pipettes). This takes into account the density of the water at the relevant temperature and the relevant air.
ISO 8655 addresses the needs of suppliers, test houses, and users and specifies the general requirements for piston-operated liquid handling devices. It is structured in seven chapters:<br />ISO 8655-1: Terminology, general requirements, and user recommendations<br />ISO 8655-2: Piston pipettes<br />ISO 8655-3: Piston burettes<br />ISO 8655-4: Dilutors<br />ISO 8655-5: Dispensers<br />ISO 8655-6: Gravimetric method for the determination of measurement error<br />ISO 8655-7: Non-gravimetric methods for assessment of equipment performance (titration, photometry)
The systematic error describes the deviation of the dispensed volume from the nominal volume or from the selected volume of the piston stroke device, and is determined by establishing the mean value of (usually) 10 measurements. The random error describes the mean variation of the dispensed volumes from the mean value of the dispensed volumes, and is determined by calculating the repeat standard deviation of (usually) 10 measurements.
The Z factor is included in EN ISO 8655-6 and was incorporated into our SOP. As we understand it, it was incorporated in the standard by the Physikalisch Technische Bundesanstalt in Braunschweig.<br />The Z factor describes the density of water at a particular temperature using a 5th degree polynomial. Furthermore, the density of water is corrected by the density of air (derived from air pressure) and a fixed correction value for balances. These corrections are the key reasons for the deviation from density values in various books of tables.
According to the EN ISO 8655 standard, a variable pipette should be calibrated to 3 volumes during conformity testing:<br />1. Nominal volume,<br />2. 50 % of nominal volume,<br />3. Smallest value that can be set or 10 % of nominal volume.
The weight fluctuations you measured would appear to be a temperature problem. Please ensure that the pipette, the distilled water used for testing, the tips etc… are all at the same temperature. It is recommended that the pipettes be placed in the weighing room at least 2 hours before the test is performed. While measuring, ensure that transfer of heat from your hands is kept to a minimum (otherwise weight fluctuations may occur!) Tip: do not constantly grasp the pipette in the palm of your hand while waiting for each measurement result! We recommend our standard operating procedure (SOP) for pipettes as a source of basic information on pipette calibration.
According to ISO 8655, all channels of multi-channel pipettes are to be tested. All channels have to be filled but only the channel to be tested is measured. Because of the high number of measurements (8-channel pipette: 240 measurements, 12-channel pipette: 360 measurements), it makes sense to send the multi-channel pipette to a professional calibration lab. These labs have special constructions for balances to ensure that all channels are filled but only one channel is measured.
To attach the Multipette holder adapter you first need to remove one pipette holder from the pipette carousel. To do this, undo the screw at the top end of the pipette holder. Once the pipette holder has been removed from the carousel you can put the Multipette holder in the free space in the carousel and screw it all firmly together again.
The lower part of the Xplorer can be autoclaved. 175 autoclaving cycles (121 °C, 1 bar, 20 min.) were tested. This number of tests corresponds to the average use over 5 years.
Yes, there are several options for adjusting the pipette in the ""Options"" operating mode, e.g. to 75 % ethanol, altitude, elongated tips. Furthermore, individual adjustments are possible, as 1-point, 2-point or 3-point settings.
A complete run with volume, speed, etc… can be saved at the program level. After exiting the program level, the run can no longer be changed just by pressing the keys. Additionally, the Xplorer plus offers possibilities of protecting settings by password.
The Xplorer is UV-resistant. Longer periods of irradiation can lead to very minimal material discoloration that does not have any effect on functionality.
The Xplorer technical data is only valid for pipetting. Conformity must be achieved for this operating mode. For piston stroke pipettes, there are no requirements for dispensing accuracy according to EN ISO 8655.
The Xplorer can be charged using the power supply included in the accessories or in the charging stand or charging carousel (optional accessories). One Xplorer can be charged in the charging stand and four in the charging carousel. A single-channel or multi-channel Xplorer can be attached to a charging shell.
No, the Xplorer cannot be charged in the Multipette charging stand. But the charging shells of the Xplorer charging stand can be exchanged. A charging shell for a Multipette stream/Xstream can be used instead of a charging shell for an Xplorer.
The lithium polymer battery features high energy density. Its service life is several years; but this is strongly dependent on usage and storage conditions.
The certificate of conformity for the Eppendorf Xplorer<sup>®</sup> system is valid for epTIPS. However, the Xplorer can also be used with common competitor tips.
In the operating mode "Pipetting and Mixing," the sample is automatically mixed with the preparation after dispensing. The mixing cycles and mixing volume can be individually defined for this.
On the Xplorer, the pipette can be adjusted to different liquids or geographic heights using 1, 2 or 3-point adjustment, taking into account the corresponding density and associated scale results. The setting for ethanol 75 %25 or glycerol 50 %25 is completed using an internally determined factor that takes into account the density.
The user decides upon the frequency of calibration, the number of test volumes and the number of measurements for each test volume within the framework of monitoring his test materials. This can vary depending on the frequency of use of the pipette, the number of users of the device, the aggressiveness of the liquids to be dispensed and the error limits deemed acceptable by the user. However, an inspection should be carried out at least once a year. See also EN ISO 8655-6.
