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Centrifuge 5427 R
Centrifuge 5427 R was designed with high-throughput research applications in mind. Choose the Centrifuge 5427 R if a 24–place microcentrifuge (e.g., Centrifuge 5424 R) doesn’t meet your needs regarding tube capacity and if you don’t need the extra versatility our cross–over Centrifuge 5430 R can offer you.
Where there is a demand for high sample throughput, many centrifuges call it a day in regards to capacity. Not so Eppendorf's new Centrifuge 5427 R – it thrives on high volume challenges.
With a new double-row 48-place rotor it delivers increased efficiency with fast running times in a safe and reliable manner.
An intuitive human interface and design concept makes for compelling user comfort.
The Centrifuge 5427 R is the ideal solution for demanding high-end research applications.
In addition, this microcentrifuge incorporates an innovative compressor technology which – by taking ecological aspects into account – tempers sensible samples precisely to guarantee a proper preparation of your analysis.
Product Features
New 48-place rotor reduces time expenditure of daily routine works significantly
Short-Spin function allows for quick short centrifugation with pre-selectable rotation speed
Eppendorf QuickLock®-system for quick rotor lid opening and closing
Very quiet operation due to further improved sound insulation
FastTemp™ for rapid pre-cooling of the centrifuge, e. g. from 23 °C to 4 °C in in aprox. 11 minutes
Continuous cooling keeps the temperature after the run constant—Your samples stay safe
Alternatively to continuous cooling: ECO-shut off of the compressor (adjustable after 1, 2, 4 or 8 hours of non-use) for a lower energy consumption
The Eppendorf microcentrifuges set the standards in terms of design and usability. This has been confirmed by receiving the external certificate »excellent! Universal Design« of the TÜV Nord organisation.
Yes, in the event of a power failure, the unit can be opened by means of the rotor key. Before the centrifuge is opened, always remove the mains plug from the plug socket and wait for the rotor to come to a standstill. For the emergency release of the centrifuge lid, the small plastic cap on the right front corner of the 5427 R has to be removed. Then insert the rotor key into the nut underneath the cap and open the lid latches by turning in anticlockwise direction.
The Centrifuge 5427 R can be cleaned with neutral detergents. For disinfection, we recommend alcohol or alcoholic disinfectants. Make sure that the relevant legal requirements and directives are observed when choosing the disinfection method.
EP" is displayed when the automatic standby function is active. If the centrifuge remains unused for more than 15 min., the standby function is activated, indicated by "EP" on the display. By using any key, the centrifuge is re-activated and ready to use.
The Centrifuge 5427 R has an automatic rotor recognition system. A newly installed rotor is recognized at the beginning of the run. The maximum speed of each rotor is automatically limited, if necessary, to prevent over-speeding of the rotors.
One advantage of centrifugation with the swing bucket rotor is the possibility of sedimenting molecules that cannot be pelletized with a fixed-angle rotor (Microgradient, e.g. blood / Ficoll).
When working with nucleic acid purification kits with spin columns by certain manufacturers, sometimes tube lids cannot be closed any more. This means you have to centrifuge with open tube lids. These tube lids might rip off during the stress of centrifugation and might damage the centrifuge. In order to prevent this, the kit rotor with the raised rotor rim was designed. Open tube lids, supported against the rim, will not rip off during centrifugation. Another feature of this rotor is the enlarged distance between the rotor bores. The enlarged distance between the bores offers a very convenient handling when loading / unloading the rotor. Especially when working with long spin columns (e. g. from Princeton Separations) you will need a larger distance between the bores to load the rotor fully and make use of the complete rotor capacity.
Due to the different rotors and adapters, a wide variety of tubes can be centrifuged with the<br />Centrifuge 5427 R: <br />• Micro test tubes (0.5 - 5 mL)<br />• PCR strips and 0.2 mL PCR tubes<br />• Spin columns (1.5/2.0 mL)<br />• Microtainer (0.6 mL
Up to 48 0.2 mL PCR tubes (or 6 x 8-tube PCR strips) can be centrifuged in the PCR rotor<br />F-45-48-5-PCR. Furthermore, 0.2 mL PCR tubes can be used in each rotor for 1.5/ 2.0 mL micro test tubes using an adapter
Spin columns or small filtration columns, for example for centrifugation of DNA or RNA, can be used in all rotors for 1.5/2.0 mL micro test tubes.<br />In particular, however, the kit rotor F-45-24-11-Kit is specially suitable for such spin columns, as open tube lids are supported by the raised edge of this rotor type and are no longer torn off (e.g. during elution of the sample from the column into a micro test tube)
The "Fast Temp" function is a temperature control run of the Centrifuge 5427 R without samples and with a rotor and temperature-specific rotational speed in order to quickly bring the rotor chamber, incl. the rotor, buckets and adapters, to the set nominal temperature. In order that the samples can be centrifuged from the beginning on at the desired temperature, one starts this temperature control run during sample preparation by pressing the "Fast Temp" key. The display shows the current temperature and the g-force (rcf) / rotational speed (rpm) as well as "FT" as symbol for a "Fast Temp" temperature run. You can find a more detailed description in the operating instructions of the Centrifuge 5427 R.
The continuous cooling starts with each rotor standstill of the Centrifuge 5427 R in order to maintain the preselected nominal temperature of the rotor chamber not only during centrifugation. To this purpose the following prerequisites must be met:<br />- The centrifuge is switched on.<br />- The centrifuge lid is closed.<br />- The nominal temperature is lower than the ambient temperature.<br />- The centrifuge is not in standby mode.<br />Continuous cooling is ended by opening the centrifuge lid or by pressing any key.
The lowest temperature of the Centrifuge 5430 R is -11 °C. The device is thereby optimized with all rotors in such a way that at least 4 °C is maintained with all rotors at the appropriate maximum speeds.
Continuous cooling is switched off if the centrifuge is not used for longer than the preset time. The centrifuge switches to standby mode.<br />- Default settings: Continuous cooling ends after 8 h.<br />- Continuous cooling can be limited to 1h, 2h or 4 h
The SOFT function of the Centrifuge 5427 R allows slower and gentler acceleration and braking compared to the normal acceleration and braking times. As a result, delicate samples are not stressed unnecessarily. In addition, the risk of the pellets being shaken up again during braking is minimised.
Firstly, the aerosol-tight rotors can be recognized by their designation abbreviation “FA” or “AT”; secondly, we have color-coded the aerosol-tight rotors.<br />All aerosol-tight rotors have a red rotor lid screw, and a red ring is printed on to the rotor shaft
Aerosol-tight rotors are always recommended or even prescribed when working with hazardous or potentially hazardous substances. These can, for example, be viruses, pathogenic bacteria, potentially infectious blood samples or radioactive sample material.
At Eppendorf, the safety of the user always has the highest priority. That is why we now fit our centrifuges with aerosol-tight rotors as standard – at no extra charge.