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GALILEO Horizontal Gel Electrophoresis Units
These horizontal gel electrophoresis units from Galileo come in a huge range of options and sizes.
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Overview
Galileo manufactures all the tools you need to make your electrophoresis research and testing more productive and much easier than ever. Feedback from researchers like you is the inspiration for making the finest life science and electrophoresis research tools in the marketplace today.
Galileo systems feature innovations like extra-wide systems for increased sample capacity and throughtput, microtiter format combs for quick sample loading, and a wide range of casting methods and optional casting Accessories for improved gel handling.
Their uncompromising requirements for quality materials and meticulous manufacturing methods ensure you of precise repeatable results, and years of flawless performance in even the most demanding laboratory conditions.
Galileo are justifiably very proud of their products and stand resolutely behind them.
Mini Gel Systems
Galileo offers three different sized mini-gel devices and two gel casting options.
- Minigel 1 units (0708 and 0911) are ideal for short, quick runs on small sample numbers.
- Minigel 2 units (1214 and 1515) are wider giving larger sample capacities.
Buffer Recirculation Options
All units can be ordered with buffer ports as an option. In addition, the 1214 unit now can be ordered with a built-in automatic buffer recirculation system, that require no additional attachments.
Gel Casting Options
These Galileo mini-gel units all feature end-gasketed RapidCast gel trays for quick, tape-free gel casting. When rotated 90 degrees from the normal run position, these trays will form a leak-proof seal against the sides of the unit’s buffer chamber. RapidCast casting units are also available for casting gels outside the unit. In addition, standard flush-cut trays featuring longer run lengths for increased sample resolution are also available for all units.
Specifications
Model |
0708 |
0911 |
1214 |
1515 |
Customisation |
Choose a standard package or customise your system with choices from the many combs and other system components. |
unit dimensions (cm) |
12 W × 17.6 L × 9.5 H |
152 W × 22 L × 9.5 H |
18 W × 24.5 L × 9.5 H |
21 W × 26 L × 9.5 H |
Gel size (cm) |
7 W × 8L |
9 W × 11L |
12 W × 14L |
15 W × 15L |
Buffer capacity |
400 mL |
600 mL |
800 mL |
1020 mL |
Gel volume (0.5 cm thick) |
28 mL |
50 mL |
84 mL |
112 mL |
Max sample capacity |
24 |
36 |
100 |
112 |
Max samples
full length run |
12 |
18 |
25 |
28 |
electrode
separation length |
18 cm |
19 cm |
22.5 cm |
24 cm |
Standard features |
Epoxy-sealed connections
Platinum electrodes
Non-slip feet |
Epoxy-sealed connections
Platinum electrodes
Non-slip feet |
Epoxy-sealed connections
Platinum electrodes
Non-slip feet |
Epoxy-sealed connections
Platinum electrodes
Non-slip feet |
Safety features |
Safety cover
Sheathed power leads |
Safety cover
Sheathed power leads |
Safety cover
Sheathed power leads |
Safety cover
Sheathed power leads |
Included tray |
RapidCast
UV transmissible
Fluorescent ruler
2 Comb slots |
RapidCast
UV transmissible
Fluorescent ruler
2 Comb slots |
RapidCast
UV transmissible
Fluorescent ruler
2 Comb slots |
RapidCast
UV transmissible
Fluorescent ruler
2 Comb slots |
Included combs |
1.5 mm, 6 & 10 well* |
1.5 mm, 10 & 14 well* |
1.5 mm, 12 & 20 well |
1.5 mm, 12 & 20 well |
Options |
Optional buffer ports |
Optional buffer ports |
Optional buffer ports or
NEW* automatic buffer recirculation
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Optional buffer ports |
Running conditions |
60 volts* |
90 volts* |
115 volts* |
120 volts* |
*Value = 5 volts/cm of electrode separation. |
Long Run Large Format Electrophoresis Systems
Galileo offers four large format sytems for researchers requiring longer run lengths and increased sample capacity. All Galileo large format units use the ExpressCast gel casting system for quick, tape-free gel casting.
Buffer Recirculation Options
The 2025 can be ordered with a built-in automatic buffer recirculation system, that requires no additional attachments. See the accessories tab for more details.
These Galileo gel units all feature the ExpressCast™ system for quick, tape-free gel casting. Standard flush-cut trays are also available for all units. See the accessories tab for more details
Specifications
Model |
Model 1325 |
Model 1515 |
Model 1525 |
Model 2025 |
Main Features |
The Galileo 1325 unit is a medium capacity system with long run lengths for high resolution. The ExpressCast™ gel casting system makes gel pouring quick, easy and tape-less! |
Extra width for increased sample thru-put. Holds up to 112 samples. |
Perfectly sized for multi-channel sample loading. |
The Galileo 2025 unit is a medium capacity system with long run lengths for high resolution.
The ExpressCast™ gel casting system makes gel pouring quick, easy and tape-less! |
Unit dimensions (cm) |
21.5 W × 37.5 L × 10.5 H |
21 W × 26 L × 9.5 H |
23.5 W × 37.5 L × 10.5 H |
29 W × 37.5 L × 10.5 H |
Gel size (cm) |
13 W × 25 L |
15 W × 15 L |
15 W × 25 L |
20 W × 25 L |
Buffer capacity |
1600 mL |
1020 mL |
1850 mL |
2300 mL |
Gel volume (0.5 cm) |
163 mL |
112 mL |
188 mL |
250 mL |
Max sample capacity |
112 |
112 |
112 |
168 |
Max samples/full length run |
28 |
28 |
28 |
42 |
Electrode
separation length
|
34.3 cm |
24 cm |
34.3 cm |
34.3 cm |
Standard features |
Epoxy-sealed connections; platinum electrodes; leveling screws & level |
Epoxy-sealed connections; platinum electrodes; non-slip feet |
Epoxy-sealed connections; platinum electrodes; leveling screws & bubble level |
Safety features |
Safety cover and sheathed power leads |
Casting system |
Galileo ExpressCast and gasketed end gates |
Included tray |
UV transmissible; fluorescent ruler; 6 comb slots |
UV transmissible; fluorescent ruler; 2 comb slots |
UV transmissible; fluorescent ruler; 4 comb slots |
UV transmissible; fluorescent ruler; 6 comb slots |
Included combs |
1.5 mm, 12, 16 & 20 well |
1.5 mm, 12 & 20 well |
Your choice |
1.5 mm, 16, 24 & 36 well* |
Options |
Optional buffer ports |
Optional buffer ports |
Optional buffer ports |
Optional buffer ports or automatic buffer recirculation |
Running conditions |
170 volts* |
120 volts* |
170 volts* |
170 volts* |
*Value = 5 volts/cm of electrode separation (constant current). |
Accessories
- Alumina cooling plate. For use with cooling systems. This plate can be sandwiched between the gel and the inner buffer chamber to improve overall temperature uniformity.
- Casting base. For quick, efficient and leak-proof casting.
Note that Galileo mini-gel accessories, including trays and combs, also fit many other gel units from other suppliers.
Automatic Buffer Recirculation
Certain Galileo horizontal electrophoresis units are now available with automatic buffer recirculation.
Galileo automatic buffer recirculation reduces detrimental ion and pH gradients in your buffer, encourages even migration of nucleic acids, and is ideal for long running gels.
Buffer recirculation can also prevent glyoxalated RNA from dissociating in the presence of ion gradients.
The system is simple and self contained.
A trapping system captures hydrogen bubbles created by electrolysis of the buffer at the cathode (-) end of the unit.
As these bubbels move up the canted recirculation tube, they carry buffer along with them and provide a gentle and reliable recirculation system.
This system is self regulating and no additional tubing or pumps are required.
Choose from three fast and efficient, tape-less casting systems from Galileo.
Gel Trays BulletRapidCast™ – cast in place system
Galileo’s RapidCast system is ideal for most small gel units with trays that are approximately equal in width and length. The dimensions of all Galileo cast-in-place gel trays are adjusted so that when the tray is placed cross- ways in the gel tank, the gasketed ends of the gel tray fit precisely against the walls of the buffer chamber. This forms a leak-proof seal for quick gel pouring. This system can also be used with RapidCast casters, for casting outside the buffer tank. A long-lived silicone rubber gasket ensures a reliable seal thru many gel castings and side grooves on all RapidCast trays provide for easy handling. All Galileo trays feature multiple comb slots, built-in fluorescent rulers and ultra-strong assembly methods for long, reliable service.
Gel Trays BulletExpressCast™ – end gated tray system
High thru-put / high resolution systems require gel trays too long to fit cross ways in the gel tank. For easy tape-less casting with these units, Galileo has developed the ExpressCast™ system. ExpressCast trays incorporate removable gasketed end gates. These end gates fit into slots at both ends of the tray, to form a quick leak-proof seal for gel casting. Because the length of ExpressCast trays is not constrained by the width of the gel unit, these trays can accommodate longer run lengths or more sample capacity. Note that for easy upgrading of your old units, Galileo ExpressCast trays are also compatible with gel units from many other manufacturers. All Galileo trays feature multiple comb slots, built-in fluorescent rulers and ultra-strong assembly methods for long, reliable service.
Gel Trays BulletMulti-Cast casters for traditional flush-cut trays
For those wishing to use traditional trays, Galileo also supplies, standard flush-cut trays for many units. The two sizes of adjustable multi-gel casting platforms from Galileo, enable quick leak-proof casting of multiple gels using flush-cut trays. These casters accomodate a wide range of tray sizes and styles, and quickly seal both ends with a simple turn of the accuation cam.
Note that for easy upgrading of your old units, Galileo flush-cut trays and multi-casters are also compatible with gel units from many other manufacturers. All Galileo trays feature multiple comb slots, built-in fluorescent rulers and ultra-strong assembly methods for long, reliable service.
Frequently Asked Questions
Agrose (Horizontal) gels
The teeth of Galileo micro-titer combs are spaced to align with 8 & 12 channel pipettes. 1 × micro-titer combs are spaced 9 mm apart on center and will align perfectly with multichannel pipettes. 2 × micro-titer combs have teeth spaced 4.5 mm apart, so that the pipette will fill every other well. Then simply by moving the pipette 1 well over (½ pipette space) the other set of wells can be filled. This sequence is repeated until all wells are filled.
When a shorter gel length is desired (usually because you have fewer samples to run) a Galileo casting dam can be inserted in the gel tray to seal the tray at any desired length.
The best way to load gels is to first make sure the fully solidified gel is submerged in the running buffer with only a thin layer of buffer over the top of the gel. While pipetting their samples into the gel, most researchers guide their pipettor with their other hand to prevent the tip from damaging the gel matrix. Since loading buffer is more dense than running buffer, the pipette tip can be held slightly above the well during filling, and the sample will flow down into the well. Be sure to slowly dispense the sample to avoid sample loss.
For uniform gels, your agarose must be must be completely dissolved and free of any particles. Remember that agarose is a long-chain polymer and takes some time to fully dissolve into solution. A good way to check your gel material is to carefully swirl it while holding it up to the light to look for any "glass-like" particles that haven't dissolved. Continue heating until the gel looks clear, like water. Remember to compensate for evaporation as continued heating will cause your gel% to increase. When the gel has been dissolved, cool it to approximately 60°C before pouring into the gel tray.
The easiest way to tell if your device is working is to look for the bubbles rising off the electrodes at each end of the device. The negative end (black) also called the cathode, should have approximately twice as many bubbles forming as at the (red) anode end. If no bubbles are forming, check that the power supply is on, in run mode, and that all connections are secure. If bubbles are forming, then current running through the system. But remember however, that proper buffer concentration and age greatly affect the elctrophoresis process. If samples are not running properly, then the most likely problem is poor buffer formulation.
The phosphate backbone of DNA gives the molecule a uniform negative charge. So it will move toward the positive anode (red) electrode.
The critical factor when comparing two devices is the inter electrode distance. This is the distance (not the length of the gel) that determines field strength. (See question below) The power supply setting is based on this value. For example: to duplicate run conditions of 5 volts/cm in two devices with 10 and 12 cm interelectrode distances, the voltage applied must be 50 and 60 volts respectively.
Remember that since the electrical field generated in the device includes the length of the buffer as well as the gel, all volts/cm calculations should be based on the distance from one gel box electrode location to the other electrode, NOT on the length of the gel. The standard run value range is 3-6 volts/cm for most DNA gels.
This can be done as long as the gel remains under buffer or distilled water, however there can be some diffusion of the DNA in the gel, so visuallizing your samples immediately will give clearer, sharper bands.
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