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DNA LoBind Tubes
DNA LoBind Tubes maximize sample recovery of nucleic acids by significantly reducing sample–to–surface binding. The ideal solution for sample preparation and long-term storage of your precious samples!.
The DNA LoBind Tube provides the genomics user with the certainty that sample loss of genetic material caused by interaction with the plastic surface of the tube will be insignificant.
With the combination of the purest selected types of polypropylene and specially developed production technologies, a tube has been developed that ensures nearly 100% recovery of DNA/RNA.
A comparison of the recovery rates of various providers shows that the DNA LoBind Tube has the lowest loss rate.
The DNA LoBind Tube is predestined for use in DNA and RNA sample preparation without loss of quality – and has been batch tested and certified PCR clean.
For the subsequent PCR we recommend the use of our thin-walled PCR tubes.
Main features
Significantly reduced DNA loss:
– < 0.3 % loss at standard reaction conditions
– < 1 % loss at high salt concentrations
(e.g. for precipitation)
Free of surface coating, e.g. silicone
Free of DNA, DNase, RNase and PCR inhibitors
Special polypropylene material manufactured under optimized conditions
All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.
For molecular-biological requirements Eppendorf offers a new standard for uniform purity specifications. All consumables marked with the "Certified PCR Clean” label are guaranteed free of human DNA, DNase, RNase and PCR inhibitors.
Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.
Yes, the batch-specific purity grades can be downloaded at our <a href="https://www.eppendorf.com/certificates" target="_blank">certificates homepage</a>.
Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.
In comparison to a Safe-Lock Tube, the DNA LoBind Tube shows significantly less DNA bonding affinity. This effect is most apparent with high salt concentrations and when working with short DNA fragments. The DNA LoBind Tube is therefore especially suitable for applications that should be carried out with the minimum loss of nucleic acid material.
The Eppendorf LoBind Tubes completely satisfy the specifications of the Eppendorf Standard Safe Lock tubes with reference to centrifugation stability (exception: Protein LoBind Tube), lid tightness, pop up safety and chemical resistance. (See Application No. 56: The best material for original Eppis.)
Not the Eppendorf LoBind Tubes are neither siliconized nor coated. The low binding affinity results from of a special type of polypropylene and the optimized manucturing process.
The Protein LoBind Tube shows, in comparison to a Safe-Lock Tube, significantly less protein binding affinity. The Protein LoBind Tube is therefore especially suitable for applications with the lowest quantities of protein.