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Protein LoBind Tubes
Eppendorf Protein LoBind Tubes are specifically designed for use in proteomics or other fields of protein research, where protein concentration tend to be very small and sample recovery is vital for assay results. Significantly more protein can be recovered for downstream analysis and enzymes remain active.
With this recently developed Eppendorf tube type, Eppendorf introduces the care-free handling of even small amounts of proteins, antibodies, viruses and peptides for the first time. Until now, tubes had to be saturated with BSA or siliconized prior to use to improve the recovery of the proteins in the plastic tube. With the new Eppendorf protein LoBind Tube this is no longer necessary.
Recovery rates of more than 97 % are now possible. The tube does not reduce enzyme activity and denaturation effects due to interaction with the surface are virtually impossible. All of these properties are achieved without the undesired side effects of a surface coating.
All LoBind tubes are batch certified PCR clean and available in a variety of sizes; each box is packaged in bags of 50 tubes to further reduce the risk of contamination.
Features
Maximum protein recovery (over 97%, BSA 1 μg/mL)
Free of surface coating, e.g. silicone
Free of DNA, DNase, RNase and ATP (PCR clean)
Special polypropylene material manufactured under optimised processing conditions
All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.
For molecular-biological requirements Eppendorf offers a new standard for uniform purity specifications. All consumables marked with the "Certified PCR Clean” label are guaranteed free of human DNA, DNase, RNase and PCR inhibitors.
Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.
Yes, the batch-specific purity grades can be downloaded at our <a href="https://www.eppendorf.com/certificates" target="_blank">certificates homepage</a>.
Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.
The Eppendorf LoBind Tubes completely satisfy the specifications of the Eppendorf Standard Safe Lock tubes with reference to centrifugation stability (exception: Protein LoBind Tube), lid tightness, pop up safety and chemical resistance. (See Application No. 56: The best material for original Eppis.)
Not the Eppendorf LoBind Tubes are neither siliconized nor coated. The low binding affinity results from of a special type of polypropylene and the optimised manucturing process.
The Protein LoBind Tube shows, in comparison to a Safe-Lock Tube, significantly less protein binding affinity. The Protein LoBind Tube is therefore especially suitable for applications with the lowest quantities of protein.