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EPPENDORF Tubes® 5.0 mL
This product represents the perfect option for working with medium-sized sample volumes. It fills the gap between existing tube formats and enables the simple and safe processing of sample volumes up to 5.0 mL.
Eppendorf Tubes are the benchmark for simple and safe sample preparation from 0.5 to 2.0 mL. For larger volumes, 15 mL and 50 mL conical tubes are available.
With medium sample volumes you may face an issue: they need to be processed with large conical screw cap tubes – impractical, inconvenient and often prone to contamination.
Eppendorf now offers the "missing link": the Eppendorf Tube 5.0 mL. This product fills the gap between existing tube versions and enables the simple and safe processing of sample volumes up to 5.0 mL!
Applications:
Easier processing of samples up to 5.0 mL
Higher yields in DNA isolation, especially with samples with limited quantity of source material
Convenient and safe cell culture applications
Easier sample access lowers the risk of contamination
Preparation of mastermixes and buffers
Less storage space needed
Safe cell and tissue lysis
Features
Ergonomic operation: Simple, practical and ergonomic single-hand operation. Large labeling area.
Lid design: Hinged lid for minimised sample evaporation during storage and incubation in a wide range of temperatures from -86 °C to 80 °C
Material: Exceptionally high-quality, transparent polypropylene, free of plasticizers, biocides or mold release agents, for reliable test results
Certified purity: Available in lot-tested and certified Eppendorf Quality, PCR clean, Sterile and Eppendorf Biopur® purity levels
Centrifugation safety: Maximum safety and stability for centrifugation up to 25,000 × g and fast and efficient protocols.
Conical shape: Compatible with accessories for conical 15 mL tubes – many existing adapters and racks can be used.
Sample recovery: Available in Eppendorf LoBind® material for maximum recovery of valuable samples
Basically, this tube type can be used for the same applications as the micro centrifugation tubes available in 1.5 and 2 mL formats. They close a gap between this small tube format and conical 15 mL tubes for sample volumes up to 5 mL. The tubes can be used in the field of cell culture. For this purpose, they are available in the purity grades “Sterile” (= sterile and pyrogen-free) or Biopur<sup>®</sup>. They are suitable for lysis of cells and tissues and purification of nucleic acids and proteins. For this, the PCR clean purity grade and LoBind variants are provided. They are also ideally suited for the preparation of large volumes of master mixes, buffers or dilution series as well as for space-saving storage of samples.
The bottom shape of the Eppendorf Tubes<sup>®</sup> 5.0 mL is similar to that of conical 15 mL tubes which means that they are compatible with a wide range of standard laboratory equipment. In addition, an extensive range of accessories is available, enabling them to be used in many devices. Both racks and storage boxes are available for lab bench work and storage. Incubation and mixing operations can be performed using suitable blocks on Eppendorf thermomixers and compatibility with Eppendorf centrifuges is ensured by special rotors and a variety of adapters. With a suitable reservoir rack module, the tubes can also be integrated into the epMotion<sup>®</sup> automated liquid handling workstation. A detailed description of the accessories is provided in Application Note No. 265
The tubes are made of high quality highly transparent polypropylene and therefore resistant to a variety of commonly used chemical substances in the laboratory. An overview is provided in Application Note No. 56.
The Eppendorf Tubes<sup>®</sup> 5.0 mL are available in the purity grades Eppendorf Quality, PCR clean and Biopur<sup>®</sup> and also in the purity grade Sterile. In addition to irradiation for sterilization, this also includes certified testing for the absence of pyrogens. The tubes are also available as DNA LoBind and Protein LoBind variants.
The Eppendorf Tubes<sup>®</sup> 5.0 mL offer secure closure at incubation temperatures up to 80 °C. For higher temperatures, the Tube Clip should be used to secure the lid.
All tube types are resistant up to 25,000 x g when used in combination with a suitable rotor or adapter. (Test conditions: 45° fixed-angle rotor at 40 °C, with aqueous salt solution (density 1.0 g/mL) and 90 mins). The mechanical loading capacity of the tubes is reduced by the use of organic solvents.