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DNA LoBind Plates
DNA LoBind Plates maximise sample recovery of nucleic acids by significantly reducing sample–to-surface binding. The ideal solution for sample preparation and long–term storage of your precious samples!
DNA LoBind plates improve recovery of nucleic acids by reducing their adsorption to the tube wall. A combination of special manufacturing technologies and selected polypropylene batches ensures nearly 100 % recovery of DNA/RNA molecules-without surface coating to eliminate the risk of sample contamination. Eppendorf DNA LoBind plates are ideal for sample preparation of long-term storage of nucleic acids in forensic, microarrays, NGS applications, and many others.
Eppendorf DNA LoBind
Products are available in tube, microplate and deepwell plate formats to meet the different application needs for various sample volumes and throughput.
DNA recovery rate
After different incubation conditions DNA recovery rate (0.2 ng/μL DNA fragement (130 bp, 32P labeled) in 2.5 M NaCl/TE buffer) in Eppendorf DNA LoBind tubes compared to tubes from different suppliers. The Eppendorf DNA LoBind Tubes revealed up to 99 % sample recovery independent of the tested incubation temperature or time.
Application Note 226:
Use of Eppendorf LoBind® Tubes to consistently prepare and store standard panels for real–time PCR absolute quantifications. Adsorption of DNA to tube surfaces effects accuracy of real–time PCR results. The much higher DNA recovery from Eppendorf® DNA LoBind tubes leads to improved standard curves and subsequently more an accurate interpretation of DNA quantity in samples assayed. Image: Flowchart of DNA standard panels preparation. Genomic DNA stock solution was serial 10–fold diluted in either competitor's low retention or Eppendorf DNA LoBind tubes. 22 μL aliquots of each dilution were stored for 24 hours at –80°C in either competitor's low retention or Eppendorf DNA LoBind Tubes before real–time PCR assay. The PCR efficiency of the samples, which were prepared and stored in Eppendorf LoBind Tubes, was much higher - see Application Note 226 for more details.
Features
Eppendorf LoBind material guarantees maximum sample recovery for improved assay results
Free of surface coating (e.g. silicone) to minimize the risk of sample interference
Lot-certified PCR clean purity grade: free of human DNA, DNase, RNase and PCR inhibitors
Available in tube, microplate, and deepwell plate formats for easy-up scaling
High-contrast Unique OptiTrack® matrix: up to 30 % faster sample identification and fewer pipetting errors
RecoverMax® well design: optimized well geometry for minimal remaining/dead volume and excellent mixing properties
Raised well rims and a smooth surface guarantee reliable sealing
Available with barcode. Additional details available at: www.eppendorf.com/barcode
All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.
For molecular-biological requirements Eppendorf offers a new standard for uniform purity specifications. All consumables marked with the "Certified PCR Clean” label are guaranteed free of human DNA, DNase, RNase and PCR inhibitors.
Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.
Yes, the batch-specific purity grades can be downloaded at our <a href="https://www.eppendorf.com/certificates" target="_blank">certificates homepage</a>.
Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.