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Protein LoBind Plates
Eppendorf Protein LoBind Plates are specifically designed for use in proteomics or other fields of protein research, where protein concentration tend to be very small and sample recovery is vital for assay results. Significantly more protein can be recovered for downstream analyses and enzymes remain active.
Eppendorf LoBind Plates maximise sample recovery by significantly reducing sample binding to the surface. A special, two-component polymer mix creates a hydrophilic surface that guarantees optimal recovery rates of valuable samples. Protein LoBind Plates have been specially designed for use in protein research or with sensitive proteomic test methods, and frequently provide significantly improved analysis results.
Eppendorf Protein LoBind products
These products are available in tube, microplate and deepwell plate formats to meet the different application needs for various sample volumes and throughput.
Significantly higher recovery rate of proteins
from Eppendorf Protein LoBind Tubes compared to other low binding tubes after incubation for 96 h. (See Application Note 180 for more details).
MALDI–TOF analysis
The use of Eppendorf Protein LoBind Tubes yield a significantly higher signal during MALDI–TOF analysis of the peptide angiotensin I when compared to the use of standard tubes. At 1 pmol, analysis becomes impossible with standard microtest tubes. The arrows identify the signals in each experiment. (Source: Dr. S. Seeber and Dr. Humeny, Institute of Biochemistry, University of Erlangen-Nürnberg, Germany, see Application Note 180 for more details).
Features
Eppendorf LoBind material guarantees maximum sample recovery for improved assay results
Free of surface coating (e.g., silicone) to minimize the risk of sample interference
Lot-certified PCR clean purity grade: free of human DNA, DNase, RNase and PCR inhibitors
Available in tube, microplate, and deepwell plate formats for easy-up scaling
High-contrast Unique OptiTrack® matrix: up to 30 % faster sample identification and fewer pipetting errors
RecoverMax® well design: optimised well geometry for minimal remaining/dead volume and excellent mixing properties
Raised well rims and a smooth surface guarantee reliable sealing in plates
Available with barcode. Additional details available at: www.eppendorf.com/barcode
Applications
Preparation or storage of protein, peptide or antibody samples
Enzymatic assays – the hydrophilic surface reduces denaturation when it comes into contact with the tube inner wall
Prevention of sample loss during storage of virus stock solutions
All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.
For molecular-biological requirements Eppendorf offers a new standard for uniform purity specifications. All consumables marked with the "Certified PCR Clean” label are guaranteed free of human DNA, DNase, RNase and PCR inhibitors.
Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.
Yes, the batch-specific purity grades can be downloaded at our <a href="https://www.eppendorf.com/certificates" target="_blank">certificates homepage</a>.
Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.