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EPPENDORF Microplates
Experience a new dimension of transparency – the Eppendorf Microplates! It has never been this easy and reliable to pipette, control and recover samples with a polypropylene microplate. A wide plate variety in line with high robustness and precision is representing the optimal solution for your application needs.
The new Eppendorf Polypropylene Microplates and Microtitre plates set new standards in terms of transparency, purity and stability!
With the easy to read OptiTrack® matrix and a clarity, unique and unbeaten for polypropylene microplates and microtitre plates, the identification of the correct plate or an individual well is easier, less error-prone and up to 30 % faster.
Do you look for clear signals in fluorescence or luminescence detection even with low concentration samples? Then the black and white Eppendorf Microplates are the right choice. They offer an excellent signal-to-noise ratio, resulting in an extremely low detection limit.
All Eppendorf Microplates are manufactured in ISO certified clean room conditions. They are available in the purity levels PCR clean (free of human DNA, DNase, RNase and PCR inhibitors) or Sterile. In accordance with the high Eppendorf quality standards, each batch is tested and certified individually.
Features
Highest transparency for better sample visibility
The unique combination of a bright border and a highcontrast alphanumeric labeling has already proved its value in Eppendorf Deepwell Plates! Now the OptiTrack® matrix well identification system makes working with microplates just as easy!
The labeling on the top side of the plate and the frame colors are as resistant to UV light and solvents as the microplates themselves. Eppendorf Microplates 384 have additional evenly spaced markings which make orientation signifi cantly easier. You can even label the frame individually with a pencil.
Superior Visual Clarity test results compared to competing products
Eppendorf's Microplates can be compared with typical plates from other manufacturers.
The reults speak for themselves in terms of the ease with which samples can be viewed with clarity and lack of colorific or detail distortion.
Test Results for Spectral Transparency and Ease of Use
The superior craftsmanship and materials use in the fabrication of Eppendorf's Polypropylene Microplates is clearly demonstrated by the results of these tests:
Test 1: Light transparency measurements The data resulting from the absorption measurements are shown in Fig. 3, expressed as light transparency. These data confi rm that transparency across the measured spectrum is higher for the Eppendorf Microplates than for the competitors’ plates.Diagram of the transparency of empty micro test plates, calculated from absorbance measurements across the spectrum of wavelengths between 350 and 650 nm.
Test 2: Timetrials conducted clearly demonstrate much faster identification of wells than possible with the conventional grid of competitors’ plates.
The average time required for marking 10 wells on one plate is 38s for Eppendorf plates, whereas for standard plates these values range between 48 and 53 s. Locating individual wells in an Eppendorf Microplate is up to 30 % faster.
The tighter grouping for Eppendorf plates demonstrates superior consistency achieved by the clearer labelling of the OptiTrack® matrix.
Labeling which is difficult to read can therefore lead to higher variability in timesensitive experiments, since well identification requires variable lengths of time.
In the case where a filled pipette tip is used for “counting up” to the correct well, the risk of contamination is highly increased.
OptiTrack® matrix for easy and fast well identification
Choice of Coloured borders: white, black
Easy-to-read, contrast-rich alphanumeric labeling
Simple well location and sample handling
UV- and Chemical-resistant labeling and color
Eppendorf's OptiTrack matrix makes work easier by providing a unique combination of colored borders with dark, contrast-rich alphanumeric labeling. This facilitates a 30% increase in sample-finding accuracy compared with other manufacturer's products.
This significantly reduces working time and mistakes and improves the efficiency of lab organisation.
The wells themselves are colorless and transparent - for a perfect view inside the wells or inspecting the underside of the plate.
g-Safe® for time saving centrifugation up to 6000 × g
Centrifugation at up to 6,000 x g without adapter in wide range of temperatures
High dimensional stability
High torsional rigidity
Suitable for swinging-bucket rotors and drum rotors
Suitable for Eppendorf MixMate® and other mixers
RecoverMax® well design for maximum sample recovery
Optimized well geometry
Reduced “wicking” and no danger of cross-contamination
No sharp-edged contours inside the well
Rounded transitions from well walls to well bottoms
Rounded well bottoms
Conical, rounded well bottoms
Significant reduction in wicking
Excellent mixing properties - speed and efficiency
Fast resuspension
Maximum recovery of sample material
The new well design features smoothed corners and transitions at the walls and at the bottom of the wells. Due to RecoverMax design the liquid collects at the lowest point at the center of the wells.
The new design prevents interfering sample deposits from accumulating in the corners, especially in rectangular wells. Benefit: considerably reduced liquid and sample losses, efficient sample mixing and wicking reduction.
For a more thorough demonstration of the practical performance of this plate design see Investigation into the impact of an optimized well design on resuspension properties, sample losses and contamination effects by Katrin Käppler-Hanno, Philip Müller and Wolf Wente of Eppendorf AG, Hamburg, Germany; Eppendorf Instrumente GmbH, Hamburg, Germany.
Flat, planar surface with raised well rims for effective sealing
The well tops have been extruded to the same depth as the rims creating a flat, planar surface which accepts a heat-seal film without creating fil-flexure thus assuring you of a reliable and robust contamination-proof seal for your samples.
Black and white microplates for improved sensitivity in fluorescence and luminescence assays
The black Eppendorf Microplates are ideal for fluorescence detection. They offer an excellent signal-to-noise ratio, resulting in an extremely low detection limit – for clear signals even with low concentration samples! The white Eppendorf Microplates are optimized for highest sensitivity in the detection of luminescence or weak fl uorescence signals, while their interfering background fl uorescence is extremely low.
Both versions are manufactured in high polished moulds from ultra-pure polypropylene. Thus unspecifi c binding of the sample, which may influence the test result at low sample concentrations, can be reduced.
High chemical resistance
The clear Eppendorf Polypropylene Microplates are ideal for storing substances or for applications requiring high resistance to solvents, such as DMSO.
Batch certified in PCR clean and Sterile quality
All Eppendorf Microplates are manufactured in ISO certi fi ed clean room conditions. They are available in the purity levels PCR clean (free of human DNA, DNase, RNase and PCR inhibitors) or Sterile. In accordance with the high Eppendorf quality standards, each batch is tested and certifi ed individually. After all, your valuable samples deserve the best treatment!
Technical Data
Eppendorf Microplate
96/F-PP
96/U-PP
96/V-PP
384/F-PP
384/V-PP
Bottom shape
Flat
Round
V-shape
Flat
V-shape
Theoretical total volume (µL)
400
360
350
150
140
Working volume (µL)
50 – 350
20 – 320
20 – 300
10 – 120
5 – 120
Position well center A1 (mm)
a/b
11.2/14.4
9.0-12.1
Distance of well center (mm)
c
9.0
4.5
Depth (mm)
11.2
11.1
11.2
11.6
11.6
Length (mm)
l
127.8
Width (mm)
w
85.5
Height (mm)
h
14.4
Base heigth (mm)
fh
6.1
Stacking height (mm)
13.0
Bottom sketches
Dimension (W × D × H)
127.8 × 85.5 × 14.4 mm Complies with standards ANSI/SBS 1-2004 to ANSI/SBS 4-2004
All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.
For molecular-biological requirements Eppendorf offers a new standard for uniform purity specifications. All consumables marked with the "Certified PCR Clean” label are guaranteed free of human DNA, DNase, RNase and PCR inhibitors.
Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.
Yes, the batch-specific purity grades can be downloaded at our <a href="https://www.eppendorf.com/certificates" target="_blank">certificates homepage</a>.
Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.
Plates made from polypropylene may be used across a broad spectrum of applications, as they are more resistant to temperature variations as well as various chemicals than plates made from polystyrene. Furthermore, they are more robust, allowing them to be centrifuged at higher g-forces. In addition, biomolecules, such as nucleic acids and proteins, bind less strongly to polypropylene.
The Eppendorf PP Microplates are made from polypropylene and are thus resistant to DMSO and other organic solvents, unlike plates made from polystyrene. An overview of chemical resistance can be found in Application Note no. 56. In case of doubt please contact Eppendorf Application Support: support@eppendorf.com.
All areas of the plate, including the colored rim, are resistant to chemicals. The alpha numeric labeling is equally durable. An overview of chemical resistance can be found in Application Note no. 56. In case of doubt please contact Eppendorf Application Support: support@eppendorf.com.
Eppendorf offers a variety of sealing options for Microplates. Self-adhesive sealers, films and foils for heat sealing, as well as re-usable sealing mats, are available. Information regarding the compatibility of the different sealing methods is listed in the Application Note no. 208.
Plates made from black material are especially well suited for fluorescence assays (e.g. PicoGreen®). The black wells prevent “cross talk” between wells and reduce background signals. The black Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in black plates. Due to carefully selected materials, the plates have very low autofluorescence. The superior signal-to-noise ratio contributes to the increased sensitivity of fluorescence assays (see Application Note no. 203).
Plates made from white material may be used for luminescence assays (chemiluminescence, scintillation proximity assays (SPA) etc.), as well as for fluorescence assays (fluorescence intensity, polarization, etc.). The white wells prevent “cross talk” between the wells while amplifying the individual signals. The white Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in white plates. Due to carefully selected materials, the plates offer maximum signal amplification. Due to their very low autofluorescence, the white Eppendorf Microplates are equally well suited for fluorescence assays. Taken together, these features allow for an improvement in the sensitivity of analyses.
Yes; however, the exact specifications of the Microplates could be compromised. The plates are available in the qualities sterile or PCR clean. Hence, autoclaving is necessary only under exceptional circumstances.
No, the LoBind quality of the Eppendorf PP Microplates is not derived from siliconization or any other type of coating. The functionality is achieved through a carefully selected material, in combination with a special manufacturing process.
Plates made from polypropylene are not suited for the standard variant, where antigen or antibodies are bound to the well wall. For this application, plates made from polystyrene are used, which have a higher binding capacity for biomolecules. However, for antibody-based fluorescence or luminescence assays (e.g. with beads) Eppendorf PP Microplates with black or white wells are suitable, respectively.
Typically, absorption measurements are not performed in plates made from polypropylene, but in plates made from polystyrene, as this material generally has a very high transparency. Since the Eppendorf PP Microplates show considerably higher transparency compared to other plates made from polypropylene (see Application Note no. 202), the Eppendorf Microplates may be used for absorption measurements in certain cases.
The Eppendorf PP Microplates are a very high quality product. In order to underline the quality, all plates are available in the degrees of purity PCR clean or Sterile. This increases the confidence and comfort of the user, who may employ the plates directly for valuable samples in sophisticated applications.
Yes, the option of Eppendorf Microplates with barcode is available. Further information can be found on the product page <a href="https://www.eppendorf.com/microplates" target="_blank">microplates</a>.
